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p cx43  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p cx43
    P Cx43, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phospho+cx43/Phospho-Connexin+43+(Ser368)+Rabbit+mAb/pm39805175-111-33-35
    Average 93 stars, based on 28 article reviews
    p cx43 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Extracellular vesicles regulate gap junction-mediated intercellular communication and HIV-1 infection of human neural progenitor cells.
    Article Snippet: .. Primary antibodies were incubated overnight at 4 ◦C in blocking buffer supplemented with 0.1% Tween-20 and applied at 1:100 (Rabbit anti-CXCR4, Abcam) or 1:1000 (rabbit anti-CD4, Abcam; mouse anti-CCR5, Abcam; mouse anti-Cx43, Abcam; rabbit anti-phospho-Cx43 [S368], Cell Signaling Technology; rabbit anti-Panx2, Abcam). .. Membranes were imaged in a LI-COR CLX imaging system after incubating membranes with the secondary antibodies diluted at 1:20,000 (anti-rabbit 800CW, anti-mouse 800CW, antirabbit 680RD, and anti-mouse 680RD, LI-COR, Lincoln, NE).

    Article Title: Improvement of astrocytic gap junction involves the anti-depressive effect of celecoxib through inhibition of NF-κB.
    Article Snippet: .. The polyvinylidene fluoride membranes were incubated with confining liquid of 5 % bovine serum albumin for 2 h at room temperature followed by incubating with the interested primary antibody at 4 °C overnight, including rabbit antiCX43 (1/1000, CST, 3512 s), mouse glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1/10000, protein tech-60004–1-Ig), rabbit antiCX30 (1/500, Abcam, ab15191), rabbit anti-phospho-CX43 (1/1000, CST, 3511 s). .. The membranes were washed with 0.1 % Tween-20 in Trisbuffered saline the next day and incubated with horseradish peroxidaseconjugated secondary and third antibodies including biotin-labeled affinity purified antibody to rabbit IgG (H+L) antibody (1/2000, Seracare, 5260–0038), biotinylated anti-mouse IgG (H+L) antibody (1/2000, Seracare, 5260–0048), streptavidin, peroxidase-labeled (1/2000, 5270–0029), peroxidase-labeled antibody to mouse IgG (produced in goat, 1/5000, Seracare, 5220–0341), peroxidase-labeled antibody to rabbit IgG (produced in goat, 1/5000, Seracare, 5220–0336) at room temperature for 2 h. Protein bands were detected using super ECL plus.

    Article Title: Extracellular vesicles regulate gap junction-mediated intercellular communication and HIV-1 infection of human neural progenitor cells
    Article Snippet: .. Primary antibodies were incubated overnight at 4 °C in blocking buffer supplemented with 0.1% Tween-20 and applied at 1:100 (Rabbit anti-CXCR4, Abcam) or 1:1000 (rabbit anti-CD4, Abcam; mouse anti-CCR5, Abcam; mouse anti-Cx43, Abcam; rabbit anti-phospho-Cx43 [S368], Cell Signaling Technology; rabbit anti-Panx2, Abcam). .. Membranes were imaged in a LI-COR CLX imaging system after incubating membranes with the secondary antibodies diluted at 1:20,000 (anti-rabbit 800CW, anti-mouse 800CW, anti-rabbit 680RD, and anti-mouse 680RD, LI-COR, Lincoln, NE).

    Article Title: Hemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection.
    Article Snippet: Protein concentrations of each sample were determined by Quantitative nucleic acid-protein Analyzer (Biomate 5; Thermo Fisher Scientific, Inc.). .. Equal load of proteins was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred electrophoretically to nitrocellulose membranes (Whatman International Ltd., Maidstone, UK), and then rocked in Tris-buffered saline containing 0.1% Tween-20 (TBST) and 5% milk at room temperature for 2 h. Membranes were incubated at 4 ̊C overnight with specific primary antibodies: rabbit anti‐Cx43 (1:1,000), rabbit anti-phospho-Cx43 (1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), and anti-GADPH antibody (1:2,000; Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China). .. The membranes were further incubated in the horseradish peroxidase-conjugated secondary antibodies (1:5,000) (Zhongshan Golden Bridge Biotechnology Co., Ltd.) for 1 h at room temperature after four rinses in TBST.

    Article Title: Hemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection
    Article Snippet: Protein concentrations of each sample were determined by Quantitative nucleic acid-protein Analyzer (Biomate 5; Thermo Fisher Scientific, Inc.). .. Equal load of proteins was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred electrophoretically to nitrocellulose membranes (Whatman International Ltd., Maidstone, UK), and then rocked in Tris-buffered saline containing 0.1% Tween-20 (TBST) and 5% milk at room temperature for 2 h. Membranes were incubated at 4°C overnight with specific primary antibodies: rabbit anti-Cx43 (1:1,000), rabbit anti-phospho-Cx43 (1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), and anti-GADPH antibody (1:2,000; Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China). .. The membranes were further incubated in the horseradish peroxidase-conjugated secondary antibodies (1:5,000) (Zhongshan Golden Bridge Biotechnology Co., Ltd.) for 1 h at room temperature after four rinses in TBST.

    Article Title: Association Between Adenosine A 2A Receptors and Connexin 43 Regulates Hemichannels Activity and ATP Release in Astrocytes Exposed to Amyloid-β Peptides.
    Article Snippet: Increasing evidence implicates astrocytes and the associated purinergic modulation in Alzheimer’s disease (AD), characterized by cognitive deficits involving the extracellular amyloid-β peptides (Aβ) accumulation.. Aβ can affect astrocytic gliotransmitters release, namely ATP, which is rapidly metabolized into adenosine by ecto-5’-nucleotidase, CD73, resulting in adenosine A2A receptors (A2AR) activation that bolsters neurodegeneration.. AD's brains exhibit an upregulation of A2AR and of connexin 43 (Cx43), which in astrocytes forms hemichannels that can mediate ATP release.

    Blocking Assay:

    Article Title: Extracellular vesicles regulate gap junction-mediated intercellular communication and HIV-1 infection of human neural progenitor cells.
    Article Snippet: .. Primary antibodies were incubated overnight at 4 ◦C in blocking buffer supplemented with 0.1% Tween-20 and applied at 1:100 (Rabbit anti-CXCR4, Abcam) or 1:1000 (rabbit anti-CD4, Abcam; mouse anti-CCR5, Abcam; mouse anti-Cx43, Abcam; rabbit anti-phospho-Cx43 [S368], Cell Signaling Technology; rabbit anti-Panx2, Abcam). .. Membranes were imaged in a LI-COR CLX imaging system after incubating membranes with the secondary antibodies diluted at 1:20,000 (anti-rabbit 800CW, anti-mouse 800CW, antirabbit 680RD, and anti-mouse 680RD, LI-COR, Lincoln, NE).

    Article Title: Extracellular vesicles regulate gap junction-mediated intercellular communication and HIV-1 infection of human neural progenitor cells
    Article Snippet: .. Primary antibodies were incubated overnight at 4 °C in blocking buffer supplemented with 0.1% Tween-20 and applied at 1:100 (Rabbit anti-CXCR4, Abcam) or 1:1000 (rabbit anti-CD4, Abcam; mouse anti-CCR5, Abcam; mouse anti-Cx43, Abcam; rabbit anti-phospho-Cx43 [S368], Cell Signaling Technology; rabbit anti-Panx2, Abcam). .. Membranes were imaged in a LI-COR CLX imaging system after incubating membranes with the secondary antibodies diluted at 1:20,000 (anti-rabbit 800CW, anti-mouse 800CW, anti-rabbit 680RD, and anti-mouse 680RD, LI-COR, Lincoln, NE).

    Western Blot:

    Article Title: Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes
    Article Snippet: The gap junction inhibitor 18α-glycyrrhetinic acid (18α-GA) and dimethyl sulfoxide (DMSO) were purchased from Sigma–Aldrich (St. Louis, MO, USA). .. The following antibodies were used: Rabbit anti-Cx43 polyclonal antibody (Cell Signaling, #3512), Rabbit anti-phospho-Cx43 (Ser368; pCx43) polyclonal antibody (Cell Signaling, #3511), Goat anti-Sost polyclonal antibody (R&D system, AF1589), Mouse anti-β-actin monoclonal antibody (A5441; Sigma–Aldrich, St. Louis, MO, USA), HRP-linked Goat anti-Rabbit IgG (Cell Signaling, #7074S), HRP-linked Rabbit anti-Goat IgG polyclonal antibody (R&D system, HAF017), HRP-linked Goat anti-Mouse IgG polyclonal antibody (AP124P; Millipore, Burlington, MA, USA), Alexa Fluor ® 594 phalloidin (A12381; ThermoFisher, Waltham, MA, USA) and Alexa Fluor ® 488 linked Goat anti-Rabbit polyclonal antibody (A11008; ThermoFisher, Waltham, MA, USA) used for Western blotting or immunofluorescence staining with MLO-Y4 osteocyte-like cells. ..

    Immunofluorescence:

    Article Title: Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes
    Article Snippet: The gap junction inhibitor 18α-glycyrrhetinic acid (18α-GA) and dimethyl sulfoxide (DMSO) were purchased from Sigma–Aldrich (St. Louis, MO, USA). .. The following antibodies were used: Rabbit anti-Cx43 polyclonal antibody (Cell Signaling, #3512), Rabbit anti-phospho-Cx43 (Ser368; pCx43) polyclonal antibody (Cell Signaling, #3511), Goat anti-Sost polyclonal antibody (R&D system, AF1589), Mouse anti-β-actin monoclonal antibody (A5441; Sigma–Aldrich, St. Louis, MO, USA), HRP-linked Goat anti-Rabbit IgG (Cell Signaling, #7074S), HRP-linked Rabbit anti-Goat IgG polyclonal antibody (R&D system, HAF017), HRP-linked Goat anti-Mouse IgG polyclonal antibody (AP124P; Millipore, Burlington, MA, USA), Alexa Fluor ® 594 phalloidin (A12381; ThermoFisher, Waltham, MA, USA) and Alexa Fluor ® 488 linked Goat anti-Rabbit polyclonal antibody (A11008; ThermoFisher, Waltham, MA, USA) used for Western blotting or immunofluorescence staining with MLO-Y4 osteocyte-like cells. ..

    Staining:

    Article Title: Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes
    Article Snippet: The gap junction inhibitor 18α-glycyrrhetinic acid (18α-GA) and dimethyl sulfoxide (DMSO) were purchased from Sigma–Aldrich (St. Louis, MO, USA). .. The following antibodies were used: Rabbit anti-Cx43 polyclonal antibody (Cell Signaling, #3512), Rabbit anti-phospho-Cx43 (Ser368; pCx43) polyclonal antibody (Cell Signaling, #3511), Goat anti-Sost polyclonal antibody (R&D system, AF1589), Mouse anti-β-actin monoclonal antibody (A5441; Sigma–Aldrich, St. Louis, MO, USA), HRP-linked Goat anti-Rabbit IgG (Cell Signaling, #7074S), HRP-linked Rabbit anti-Goat IgG polyclonal antibody (R&D system, HAF017), HRP-linked Goat anti-Mouse IgG polyclonal antibody (AP124P; Millipore, Burlington, MA, USA), Alexa Fluor ® 594 phalloidin (A12381; ThermoFisher, Waltham, MA, USA) and Alexa Fluor ® 488 linked Goat anti-Rabbit polyclonal antibody (A11008; ThermoFisher, Waltham, MA, USA) used for Western blotting or immunofluorescence staining with MLO-Y4 osteocyte-like cells. ..

    Nucleic Acid Electrophoresis:

    Article Title: Hemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection.
    Article Snippet: Protein concentrations of each sample were determined by Quantitative nucleic acid-protein Analyzer (Biomate 5; Thermo Fisher Scientific, Inc.). .. Equal load of proteins was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred electrophoretically to nitrocellulose membranes (Whatman International Ltd., Maidstone, UK), and then rocked in Tris-buffered saline containing 0.1% Tween-20 (TBST) and 5% milk at room temperature for 2 h. Membranes were incubated at 4 ̊C overnight with specific primary antibodies: rabbit anti‐Cx43 (1:1,000), rabbit anti-phospho-Cx43 (1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), and anti-GADPH antibody (1:2,000; Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China). .. The membranes were further incubated in the horseradish peroxidase-conjugated secondary antibodies (1:5,000) (Zhongshan Golden Bridge Biotechnology Co., Ltd.) for 1 h at room temperature after four rinses in TBST.

    Article Title: Hemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection
    Article Snippet: Protein concentrations of each sample were determined by Quantitative nucleic acid-protein Analyzer (Biomate 5; Thermo Fisher Scientific, Inc.). .. Equal load of proteins was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred electrophoretically to nitrocellulose membranes (Whatman International Ltd., Maidstone, UK), and then rocked in Tris-buffered saline containing 0.1% Tween-20 (TBST) and 5% milk at room temperature for 2 h. Membranes were incubated at 4°C overnight with specific primary antibodies: rabbit anti-Cx43 (1:1,000), rabbit anti-phospho-Cx43 (1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), and anti-GADPH antibody (1:2,000; Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China). .. The membranes were further incubated in the horseradish peroxidase-conjugated secondary antibodies (1:5,000) (Zhongshan Golden Bridge Biotechnology Co., Ltd.) for 1 h at room temperature after four rinses in TBST.

    Saline:

    Article Title: Hemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection.
    Article Snippet: Protein concentrations of each sample were determined by Quantitative nucleic acid-protein Analyzer (Biomate 5; Thermo Fisher Scientific, Inc.). .. Equal load of proteins was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred electrophoretically to nitrocellulose membranes (Whatman International Ltd., Maidstone, UK), and then rocked in Tris-buffered saline containing 0.1% Tween-20 (TBST) and 5% milk at room temperature for 2 h. Membranes were incubated at 4 ̊C overnight with specific primary antibodies: rabbit anti‐Cx43 (1:1,000), rabbit anti-phospho-Cx43 (1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), and anti-GADPH antibody (1:2,000; Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China). .. The membranes were further incubated in the horseradish peroxidase-conjugated secondary antibodies (1:5,000) (Zhongshan Golden Bridge Biotechnology Co., Ltd.) for 1 h at room temperature after four rinses in TBST.

    Article Title: Hemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection
    Article Snippet: Protein concentrations of each sample were determined by Quantitative nucleic acid-protein Analyzer (Biomate 5; Thermo Fisher Scientific, Inc.). .. Equal load of proteins was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred electrophoretically to nitrocellulose membranes (Whatman International Ltd., Maidstone, UK), and then rocked in Tris-buffered saline containing 0.1% Tween-20 (TBST) and 5% milk at room temperature for 2 h. Membranes were incubated at 4°C overnight with specific primary antibodies: rabbit anti-Cx43 (1:1,000), rabbit anti-phospho-Cx43 (1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), and anti-GADPH antibody (1:2,000; Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China). .. The membranes were further incubated in the horseradish peroxidase-conjugated secondary antibodies (1:5,000) (Zhongshan Golden Bridge Biotechnology Co., Ltd.) for 1 h at room temperature after four rinses in TBST.



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    Image Search Results


    Mice were infected intranasally with 5×10 5 i.u. MAdV-3 or saline (mock) and cardiac tissue was harvested 7 d.p.i. for analyses. A) RT-qPCR of heart tissue mRNA for major cardiac gap junction genes Gja1, Gjc1 , and Gja5 . B) Confocal immunofluorescence microscopy from cryosections to detect Cx43 (green) and N-cadherin (red), with quantification of fluorescent intensity line scans across intercalated discs on right (scale bar: 50 μm). C) Western blotting of cardiac ventricle tissue probed for Cx43, with GAPDH as loading control to normalize quantitation on right. D) Western blotting of cardiac ventricle tissue probed for phospho-Cx43 Ser368 with quantification normalized to total Cx43 on right. *p ≤ 0.05, **p ≤ 0.01, student’s t-test. Data are represented as mean ± SEM.

    Journal: bioRxiv

    Article Title: Acute adenoviral cardiac infection elicits an arrhythmogenic substrate prior to inflammatory myocardial remodeling and myocarditis

    doi: 10.1101/2022.11.29.518421

    Figure Lengend Snippet: Mice were infected intranasally with 5×10 5 i.u. MAdV-3 or saline (mock) and cardiac tissue was harvested 7 d.p.i. for analyses. A) RT-qPCR of heart tissue mRNA for major cardiac gap junction genes Gja1, Gjc1 , and Gja5 . B) Confocal immunofluorescence microscopy from cryosections to detect Cx43 (green) and N-cadherin (red), with quantification of fluorescent intensity line scans across intercalated discs on right (scale bar: 50 μm). C) Western blotting of cardiac ventricle tissue probed for Cx43, with GAPDH as loading control to normalize quantitation on right. D) Western blotting of cardiac ventricle tissue probed for phospho-Cx43 Ser368 with quantification normalized to total Cx43 on right. *p ≤ 0.05, **p ≤ 0.01, student’s t-test. Data are represented as mean ± SEM.

    Article Snippet: Primary antibodies were incubated overnight at 4 °C utilizing: rabbit anti-Cx43 (1:3,000; Sigma-Aldrich), mouse anti-GAPDH (1:2,000; Santa Cruz Biotechnology), and rabbit anti-phospho-Cx43 Ser368 [D6W8P] (1:1,000; Cell Signaling Technology).

    Techniques: Infection, Saline, Quantitative RT-PCR, Immunofluorescence, Microscopy, Western Blot, Control, Quantitation Assay

    HiPSC-CMs were infected with HAdV-LacZ or HAdV-5 at an MOI 10 and harvested 24 h.p.i. A) Western blotting of cells probed for phospho-Cx43 Ser368 with quantification normalized to total Cx43 on right (n=3). B) Confocal immunofluorescence microscopy from fixed HiPSC-CMs to detect nuclei (blue) and Human Adenoviral protein E1A (red) (scale bar: 25 μm). C) Live-cell confocal microscopy 24 h.p.i. using Fluo-4 AM to measure Ca 2+ transients and quantification of fluorescence signaling over time in one field of view (scale bar: 25 μm). D) Average peak-to-peak fluorescence intensity variance measured per cell over time (n=9). *p ≤ 0.05, **p 0.01, student’s t-test. Data are represented as mean ± SEM.

    Journal: bioRxiv

    Article Title: Acute adenoviral cardiac infection elicits an arrhythmogenic substrate prior to inflammatory myocardial remodeling and myocarditis

    doi: 10.1101/2022.11.29.518421

    Figure Lengend Snippet: HiPSC-CMs were infected with HAdV-LacZ or HAdV-5 at an MOI 10 and harvested 24 h.p.i. A) Western blotting of cells probed for phospho-Cx43 Ser368 with quantification normalized to total Cx43 on right (n=3). B) Confocal immunofluorescence microscopy from fixed HiPSC-CMs to detect nuclei (blue) and Human Adenoviral protein E1A (red) (scale bar: 25 μm). C) Live-cell confocal microscopy 24 h.p.i. using Fluo-4 AM to measure Ca 2+ transients and quantification of fluorescence signaling over time in one field of view (scale bar: 25 μm). D) Average peak-to-peak fluorescence intensity variance measured per cell over time (n=9). *p ≤ 0.05, **p 0.01, student’s t-test. Data are represented as mean ± SEM.

    Article Snippet: Primary antibodies were incubated overnight at 4 °C utilizing: rabbit anti-Cx43 (1:3,000; Sigma-Aldrich), mouse anti-GAPDH (1:2,000; Santa Cruz Biotechnology), and rabbit anti-phospho-Cx43 Ser368 [D6W8P] (1:1,000; Cell Signaling Technology).

    Techniques: Infection, Western Blot, Immunofluorescence, Microscopy, Confocal Microscopy, Fluorescence