p cx43 (Cell Signaling Technology Inc)
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P Cx43, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+phospho+cx43/Phospho-Connexin+43+(Ser368)+Rabbit+mAb/pm39805175-111-33-35
Average 93 stars, based on 28 article reviews
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Incubation:Article Title: Extracellular vesicles regulate gap junction-mediated intercellular communication and HIV-1 infection of human neural progenitor cells. Article Snippet: .. Primary antibodies were incubated overnight at 4 ◦C in blocking buffer supplemented with 0.1% Tween-20 and applied at 1:100 (Rabbit anti-CXCR4, Abcam) or 1:1000 (rabbit anti-CD4, Abcam; mouse anti-CCR5, Abcam; mouse anti-Cx43, Abcam; Article Title: Improvement of astrocytic gap junction involves the anti-depressive effect of celecoxib through inhibition of NF-κB. Article Snippet: .. The polyvinylidene fluoride membranes were incubated with confining liquid of 5 % bovine serum albumin for 2 h at room temperature followed by incubating with the interested primary antibody at 4 °C overnight, including rabbit antiCX43 (1/1000, CST, 3512 s), mouse glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1/10000, protein tech-60004–1-Ig), rabbit antiCX30 (1/500, Abcam, ab15191), Article Title: Extracellular vesicles regulate gap junction-mediated intercellular communication and HIV-1 infection of human neural progenitor cells Article Snippet: .. Primary antibodies were incubated overnight at 4 °C in blocking buffer supplemented with 0.1% Tween-20 and applied at 1:100 (Rabbit anti-CXCR4, Abcam) or 1:1000 (rabbit anti-CD4, Abcam; mouse anti-CCR5, Abcam; mouse anti-Cx43, Abcam; Article Title: Hemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection. Article Snippet: Protein concentrations of each sample were determined by Quantitative nucleic acid-protein Analyzer (Biomate 5; Thermo Fisher Scientific, Inc.). .. Equal load of proteins was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred electrophoretically to nitrocellulose membranes (Whatman International Ltd., Maidstone, UK), and then rocked in Tris-buffered saline containing 0.1% Tween-20 (TBST) and 5% milk at room temperature for 2 h. Membranes were incubated at 4 ̊C overnight with specific primary antibodies: rabbit anti‐Cx43 (1:1,000), Article Title: Hemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection Article Snippet: Protein concentrations of each sample were determined by Quantitative nucleic acid-protein Analyzer (Biomate 5; Thermo Fisher Scientific, Inc.). .. Equal load of proteins was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred electrophoretically to nitrocellulose membranes (Whatman International Ltd., Maidstone, UK), and then rocked in Tris-buffered saline containing 0.1% Tween-20 (TBST) and 5% milk at room temperature for 2 h. Membranes were incubated at 4°C overnight with specific primary antibodies: rabbit anti-Cx43 (1:1,000), Article Title: Association Between Adenosine A 2A Receptors and Connexin 43 Regulates Hemichannels Activity and ATP Release in Astrocytes Exposed to Amyloid-β Peptides. Article Snippet: Increasing evidence implicates astrocytes and the associated purinergic modulation in Alzheimer’s disease (AD), characterized by cognitive deficits involving the extracellular amyloid-β peptides (Aβ) accumulation.. Aβ can affect astrocytic gliotransmitters release, namely ATP, which is rapidly metabolized into adenosine by ecto-5’-nucleotidase, CD73, resulting in adenosine A2A receptors (A2AR) activation that bolsters neurodegeneration.. AD's brains exhibit an upregulation of A2AR and of connexin 43 (Cx43), which in astrocytes forms hemichannels that can mediate ATP release. Blocking Assay:Article Title: Extracellular vesicles regulate gap junction-mediated intercellular communication and HIV-1 infection of human neural progenitor cells. Article Snippet: .. Primary antibodies were incubated overnight at 4 ◦C in blocking buffer supplemented with 0.1% Tween-20 and applied at 1:100 (Rabbit anti-CXCR4, Abcam) or 1:1000 (rabbit anti-CD4, Abcam; mouse anti-CCR5, Abcam; mouse anti-Cx43, Abcam; Article Title: Extracellular vesicles regulate gap junction-mediated intercellular communication and HIV-1 infection of human neural progenitor cells Article Snippet: .. Primary antibodies were incubated overnight at 4 °C in blocking buffer supplemented with 0.1% Tween-20 and applied at 1:100 (Rabbit anti-CXCR4, Abcam) or 1:1000 (rabbit anti-CD4, Abcam; mouse anti-CCR5, Abcam; mouse anti-Cx43, Abcam; Western Blot:Article Title: Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes Article Snippet: The gap junction inhibitor 18α-glycyrrhetinic acid (18α-GA) and dimethyl sulfoxide (DMSO) were purchased from Sigma–Aldrich (St. Louis, MO, USA). .. The following antibodies were used: Rabbit anti-Cx43 polyclonal antibody (Cell Signaling, #3512), Immunofluorescence:Article Title: Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes Article Snippet: The gap junction inhibitor 18α-glycyrrhetinic acid (18α-GA) and dimethyl sulfoxide (DMSO) were purchased from Sigma–Aldrich (St. Louis, MO, USA). .. The following antibodies were used: Rabbit anti-Cx43 polyclonal antibody (Cell Signaling, #3512), Staining:Article Title: Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes Article Snippet: The gap junction inhibitor 18α-glycyrrhetinic acid (18α-GA) and dimethyl sulfoxide (DMSO) were purchased from Sigma–Aldrich (St. Louis, MO, USA). .. The following antibodies were used: Rabbit anti-Cx43 polyclonal antibody (Cell Signaling, #3512), Nucleic Acid Electrophoresis:Article Title: Hemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection. Article Snippet: Protein concentrations of each sample were determined by Quantitative nucleic acid-protein Analyzer (Biomate 5; Thermo Fisher Scientific, Inc.). .. Equal load of proteins was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred electrophoretically to nitrocellulose membranes (Whatman International Ltd., Maidstone, UK), and then rocked in Tris-buffered saline containing 0.1% Tween-20 (TBST) and 5% milk at room temperature for 2 h. Membranes were incubated at 4 ̊C overnight with specific primary antibodies: rabbit anti‐Cx43 (1:1,000), Article Title: Hemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection Article Snippet: Protein concentrations of each sample were determined by Quantitative nucleic acid-protein Analyzer (Biomate 5; Thermo Fisher Scientific, Inc.). .. Equal load of proteins was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred electrophoretically to nitrocellulose membranes (Whatman International Ltd., Maidstone, UK), and then rocked in Tris-buffered saline containing 0.1% Tween-20 (TBST) and 5% milk at room temperature for 2 h. Membranes were incubated at 4°C overnight with specific primary antibodies: rabbit anti-Cx43 (1:1,000), Saline:Article Title: Hemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection. Article Snippet: Protein concentrations of each sample were determined by Quantitative nucleic acid-protein Analyzer (Biomate 5; Thermo Fisher Scientific, Inc.). .. Equal load of proteins was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred electrophoretically to nitrocellulose membranes (Whatman International Ltd., Maidstone, UK), and then rocked in Tris-buffered saline containing 0.1% Tween-20 (TBST) and 5% milk at room temperature for 2 h. Membranes were incubated at 4 ̊C overnight with specific primary antibodies: rabbit anti‐Cx43 (1:1,000), Article Title: Hemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection Article Snippet: Protein concentrations of each sample were determined by Quantitative nucleic acid-protein Analyzer (Biomate 5; Thermo Fisher Scientific, Inc.). .. Equal load of proteins was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred electrophoretically to nitrocellulose membranes (Whatman International Ltd., Maidstone, UK), and then rocked in Tris-buffered saline containing 0.1% Tween-20 (TBST) and 5% milk at room temperature for 2 h. Membranes were incubated at 4°C overnight with specific primary antibodies: rabbit anti-Cx43 (1:1,000), |
